Part:BBa_J70034

A BioScaffold Part (Uses AarI) for Library Vector Preparation (see Part Design page)

When placed in a BioBrick assembly and cut with the restriction enzyme AarI, this part will direct the restriction enzyme to cut within the BioBrick scar

still in testing, uses AarI (2) XmaI (1)

See part design page or BBFRFC15, for details about use Note that the prefix for this is slightly different than the part described in BBF RFC 15 (due to the prefix)

Oligos for insertion into a EcoRI/SpeI cut vector such as pSB2K4:

    AATTCGCGGCCGCTTCTAGAG | GCAGGTGGACAAGAGGAGT   CCCGGGAGCTGGAACTCCCACCTGCATA (ctag)
        G                                       |                           at  gatg
                            gcaggtggacaagaggagt   cccgggagctggaactcccacctgca
   gaattcgcggccgcttctagag   J70034 sequence                                 ta  ctagtagcggccgctgcag
   BioBricks Prefix                                                         BioBricks Suffix

Forward 1: AATTCGCGGCCGCTTCTAGAG

Forward 2: GCAGGTGGACAAGAGGAGTCCCGGGAGCTGGAACTCCCACCTGCATA

Reverse 1: ACTCCTCTTGTCCACCTGCCTCTAGAAGCGGCCGCG

Reverse 2: CTAGTATGCAGGTGGGAGTTCCAGCTCCCGGG

Now have pSB2K4 plasmids containing this part, AarI and XmaI.

3/18/9: We now have a sequenced version of the part "J70034final-pSB2K4" (from J70034 1b in DNA sequence page) and 
are testing the functionality of the part (whether it can be used in a "BioScaffold parts" context)  


    AATTCGCGGCCGC^TTCT AGAG | GCAGGTGGACAAGAGGAGT   CCCGGGAGCTGGAACTCC CACCTGC Atac^tagt agcggccgctgcag
    TTAAGCGCCGGCG AAGA^TCTC                                                     atg atca^tcgccggcgacgtc

Try using this as an opportunity to make J70040 
                  TTCT AGAG   gcaggtggacacctagggtcccgggagcaccaggtcccacctgcatac(tagt)
                       TCTC                                                atg atca
Forward J70034TP.J70040.1.F: TTCTAGAGgcaggtggacacctagggtcccgggagcaccaggtcccacctgcatac
Reverse J70034TP.J70040.1.R: actagtatgcaggtgggacctggtgctcccgggaccctaggtgtccacctgcCTCT

Try using this as an opportunity to make J70010-4bp (don't make, this will not be useful)
          gagagctggttcaccaggttaattaattaattaaacctggtgaacgtggtctc
          gagagctggttcaccaggttaattaattaa    acctggtgaacgtggtctc 
TTCT AGAG gagagctggttcaccaggttaattaattaa    acctggtgaacgtggtctc tac(tagt)
     tctc                                                           atac
Forward J70034TP.J70010-4bp.1.F: TTCTAGAGgagagctggttcaccaggttaattaattaaacctggtgaacgtggtctctac
Reverse J70034TP.J70010-4bp.1.R: actagtagagaccacgttcaccaggtttaattaattaacctggtgaaccagctctcCTCT

Try using this as an opportunity to make J70030
         TTCT AGAG ctggttcacctggttaattaattaattaaaccaggtgaacgtggtctc tac(tagt)
              TCTC                                                  atg atca
Forward J70034TP.J70030.1.F: TTCTAGAGctggttcacctggttaattaattaattaaaccaggtgaacgtggtctctac
Reverse J70034TP.J70030.1.R: actagtagagaccacgttcacctggtttaattaattaattaaccaggtgaaccagCTCT

3/31/9

gcaggtggacaagaggagtcccgggagctggaactcccacctgca
gcaggtggacaagaggagtaaa       aaaggaactcccacctgca
         62.5                                           61.5  

J70034.r.pcr reverse: tttactcctcttgtccacctgc J70034.f.pcr forward: aaaggaactcccacctgca

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]